Human C5a/Complement C5a ELISA Kit

Made by Assay Genie, this Sandwich ELISA kit enables quantitative measurement of human C5a, also known as Complement Component 5a, in research samples. Built on a double-antibody sandwich format with colorimetric detection, it delivers a sensitivity of 46.875 pg/mL across a standard range of 78.125–5000 pg/mL, making it suitable for studies of inflammation and immune response where accurate quantitation of complement activation is required.

The kit has been validated for use with human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Linearity and recovery have been assessed across serum, EDTA plasma, and heparin plasma, with reported average recoveries of 96% in serum, 95% in EDTA plasma, and 100% in heparin plasma. Unopened components should be stored at 2–8 °C and remain stable for 12 months under those conditions.

The assay workflow follows a standard sandwich ELISA procedure: equilibrate reagents and TMB substrate, load standards and samples for a 90-minute primary incubation at 37 °C, add a biotin-labelled detection antibody for 60 minutes at 37 °C, incubate with HRP-Streptavidin for 30 minutes at 37 °C, develop colour with TMB substrate in the dark for 10–20 minutes, and read absorbance at 450 nm after adding the stop solution. Researchers investigating autoimmune disease, sepsis, or allergic inflammation can use this kit to explore C5a-driven mechanisms and potential therapeutic targets.