Human C3c (Complement Fragment 3c) ELISA Kit (HUES01957)

From Assay Genie, the assay is built around a biotinylated detection antibody and HRP-Streptavidin signal generation, with TMB as the chromogenic substrate; absorbance is read at 450 nm on a standard microplate reader after addition of a stop solution. Typical sample volumes are small (commonly around 5–100 µL depending on matrix), and a complete run, excluding equilibration and sample preparation, is generally completed in a few hours. Quantification relies on a lyophilized standard curve, allowing interpolation of unknown C3c concentrations directly in ng/mL. The antibodies used show no significant cross-reactivity with closely related complement fragments such as intact C3, C3a, C3b, iC3b, or C3d, supporting selective detection of the C3c cleavage product in complex samples. Researchers commonly deploy this kit in studies of C3 glomerulopathy, lupus nephritis, atypical hemolytic uremic syndrome, sepsis-associated complement consumption, complement activation profiling in COVID-19 and other infectious disease cohorts, and biomarker work in immune-complex disease. Reconstituted standards and diluted samples should be aliquoted to limit freeze-thaw cycles, and all reagents should be equilibrated to room temperature before use.