Human C2 PharmaGenie ELISA Kit (SBRS0331)

Complement component C2 plays a central role in the classical activation pathway of the human immune system, where it is cleaved by the C1 complex into the catalytic C2a fragment and the anchoring C2b fragment that assemble the C3 convertase C4b2a. This microplate-format ELISA kit from Assay Genie enables quantitative measurement of human C2 in research samples, supporting immunology, complement biology, and drug discovery laboratories.

C2 quantification is widely applied to studies of systemic lupus erythematosus, recurrent pyogenic infections, hereditary angioedema, and other complement-mediated disorders where classical pathway activity must be characterized. The kit accepts cell culture supernatant, plasma, and serum specimen types, covering both in vitro experimental models and clinical research samples. Each kit provides one complete microplate along with all reagents required for absorbance-based colorimetric readout.

Reagents should be kept refrigerated until first use and equilibrated to room temperature before the assay workflow begins; storage temperature and shelf-life details are listed on the product insert. The protocol is intended for research use only and is not validated for in vitro diagnostic procedures. Assay Genie validates the reagents for specific detection of human C2 with minimal cross-reactivity in the indicated matrices.

Investigators comparing classical pathway activation across patient cohorts, screening compound libraries for complement modulators, or profiling C2 secretion from cultured human cell lines will find the workflow well matched to moderate-throughput studies in academic and pharmaceutical settings.