Human C13orf33/Uncharacterized protein C13orf33 ELISA Kit

This Sandwich ELISA from Assay Genie is designed for the quantitative detection of human C13orf33 (also known as MEDAG, MEDA-4, or hAWMS3), an uncharacterized protein linked to adipogenesis. The assay uses a double-antibody, colorimetric format and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a working standard range of 78.125-5000 pg/mL and a sensitivity of 46.875 pg/mL.

Performance data supplied by the manufacturer cover linearity and spike-and-recovery across serum, EDTA plasma and heparin plasma at 1:2, 1:4 and 1:8 dilutions; recovery ranges fall between 87-104% across the three matrices, with reported averages of 98% (serum), 92% (EDTA plasma) and 96% (heparin plasma). The procedure involves a 37°C primary incubation, biotinylated detection antibody binding, HRP-streptavidin binding, TMB colour development and a final absorbance read at 450 nm, and the kit is intended for storage at 2-8°C for up to 12 months.