Human C-X-C motif chemokine 13 (CXCL13) ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the quantitative measurement of human C-X-C motif chemokine 13 (CXCL13) in biological samples. The kit is for research use only and recognises both natural and recombinant human CXCL13 with reported reactivity in human samples.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Performance data quoted in the product literature includes a detection range of 7.8-500 pg/mL and a sensitivity of 3.2 pg/mL. Intra-assay precision is given as 5.9% CV and inter-assay precision as 8.3% CV. Spike-and-recovery values are reported for serum (average 103%, range 97-109%) and plasma (average 105%, range 99-111%), and linearity ranges are provided for serum, EDTA plasma and heparin plasma at dilutions of 1:2, 1:4, 1:8 and 1:16.

CXCL13 is described in the source material as a chemokine that is chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes or neutrophils, does not induce calcium release in B-lymphocytes, and binds to BLR1/CXCR5. The protein is secreted, has a reported molecular weight of 12,664 Da, and is associated with the UniProt entry O43927. Synonyms listed include Angie, B cell-attracting chemokine 1, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, BCA-1, BCA1, BLC and SCYB13.