Human C Reactive Protein (CRP) ELISA Kit

C-Reactive Protein (CRP) is a pentameric acute-phase reactant synthesized by hepatocytes in response to inflammatory cytokines such as IL-6. Because circulating CRP concentrations rise rapidly during infection, tissue injury, and chronic inflammatory states, quantitative CRP measurement supports immunology research, cardiovascular risk studies, sepsis investigations, and drug-response monitoring. This in vitro kit from Assay Genie enables sensitive quantification of human CRP across diverse experimental matrices.

The assay uses a matched pair of antibodies on a pre-coated strip plate, producing a chromogenic endpoint proportional to CRP captured from the specimen. Validation supports its use with serum-based samples, plasma-derived specimens, tissue homogenates, cell lysates, urine, cerebrospinal fluid, and cell culture supernatants, giving researchers flexibility to study systemic inflammation, localized tissue responses, or cell-based models. Investigators commonly apply it to track inflammatory kinetics, stratify patient cohorts, or evaluate candidate anti-inflammatory compounds.

Each kit ships as a 96-assay configuration and is intended for research use only. Components are stored refrigerated until first use; reconstituted reagents and standards should be kept cold during the run and returned to the recommended storage condition afterward. The chromogenic signal is read on a standard microplate reader, and CRP concentrations are interpolated from a standard curve. The 21 kDa subunit of the pentameric protein is specifically recognized, supporting clean discrimination from related pentraxins such as serum amyloid P component.