Human C-Peptide PharmaGenie ELISA Kit (SBRS0020)

C-Peptide is a 31-amino acid polypeptide cleaved from proinsulin during endogenous insulin biosynthesis, making it a key biomarker for assessing pancreatic beta-cell function. This competitive immunoassay kit from Assay Genie provides quantification of C-Peptide in human serum and cell culture supernatant samples, supporting diabetes research workflows and metabolic studies.

The kit includes all reagents required to run a complete 96-assay microplate experiment, delivering a colorimetric readout on a standard laboratory plate reader. Because the assay uses a competitive format, it is well matched to detecting a small peptide analyte and tolerates the dynamic range observed with clinical and preclinical research samples.

Typical applications include evaluating endogenous insulin secretion in Type 1 and Type 2 diabetes studies, monitoring beta-cell preservation in islet transplant follow-up, and screening cell culture models of insulinoma or stem-cell-derived beta cells. Researchers can apply the same protocol to human serum specimens and to conditioned media from cultured islets or beta-cell lines, enabling cross-sample comparison within a study.

The kit ships as a complete in vitro research-use-only package with pre-coated wells and lyophilised standards, reagents, and a detailed protocol. Each lot is validated by the manufacturer for intra- and inter-assay precision, and the included step-by-step protocol guides both experienced immunoassay users and laboratories new to competitive ELISA workflows.