Human Butyrophilin subfamily 2 member A1 (BTN2A1) ELISA Kit

Assay Genie offers a sandwich-format ELISA for the quantitative measurement of Butyrophilin subfamily 2 member A1 (BTN2A1) in human samples. The kit accepts serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with reactivity confirmed for human specimens. BTN2A1 is a member of the butyrophilin family of proteins implicated in immune regulation and T cell activation, making it of interest for immunological and biomarker research.

Reported performance characteristics include a detection range of 0.312–20 ng/mL and a sensitivity of 0.12 ng/mL. Intra-assay precision is 6.9% CV and inter-assay precision is 8.8% CV. Linearity has been demonstrated across dilutions from 1:2 to 1:16 in serum (91–120%), EDTA plasma (100–117%) and heparin plasma (92–109%). Average spike-and-recovery values are 103% in serum (range 97–109%) and 105% in plasma (range 99–111%). The kit is specific for natural and recombinant human BTN2A1, with listed synonyms BT2.1 and BTF1.

BTN2A1 is an integral single-pass type I membrane protein encoded within a cluster of butyrophilin-like genes on chromosome 6 in the immunoglobulin superfamily, and is associated with cardiovascular research. The product is intended for research use only.