Human BTNL8 (Butyrophilin-like protein 8) ELISA Kit (HUFI04607)

Researchers studying mucosal immunity, inflammatory bowel disease, and gut barrier function can use this Assay Genie ELISA Kit to quantitatively measure Butyrophilin-like protein 8 (BTNL8) in human specimens. BTNL8 belongs to the butyrophilin family of immunoglobulin-like molecules and is expressed predominantly in intestinal epithelial cells, where it modulates gamma-delta T-cell activation and contributes to mucosal immune homeostasis. Altered BTNL8 expression has been reported in ulcerative colitis and Crohn's disease, positioning the protein as a candidate biomarker in gastroenterology and mucosal immunology research.

Built around a matched antibody pair capture format, the assay uses antibody-coated microplate wells, a biotinylated detection reagent, and an enzyme-mediated substrate reaction that produces a measurable optical signal on a standard plate reader. The kit is supplied as a 96 Assays configuration with pre-coated plate, reference standards, dilution buffer, wash concentrate, detection antibody, streptavidin-enzyme conjugate, substrate solution, and stop reagent. The protocol accommodates human serum-type samples and plasma-type preparations, and can be adapted for cell culture supernatants and tissue lysates following appropriate validation by the investigator.

Assay Genie supports the product with a detailed insert covering standard curve preparation, working dilution recommendations, and performance characteristics. Reagents should be stored at the temperatures specified in the product documentation; allow refrigerated components to reach room temperature before use and protect light-sensitive solutions, particularly the substrate and conjugate working solutions, from prolonged exposure to direct light. The kit is labeled for research use only and is not intended for clinical diagnostic decision-making.