Human BRWD1 (Bromodomain and WD repeat-containing protein 1) ELISA KIT (HUFI05404)

The Assay Genie BRWD1 immunoassay kit is a microplate-based, in vitro research-use-only reagent configured to support quantitative measurement of Bromodomain and WD repeat-containing protein 1. BRWD1 functions as a chromatin-associated bromodomain reader that recognizes acetylated histone tails and links these chromatin states to transcriptional regulation, with documented roles in spermatogenesis, germ cell maturation, and X-linked intellectual disability. This kit is therefore relevant to studies of epigenetic remodeling, male reproductive biology, and neurodevelopmental gene regulation.

Supplied components typically include a pre-coated capture microplate, biotinylated detection antibody, streptavidin-HRP conjugate, lyophilized reference standard, and assay diluent. Working reagents should be refrigerated at 4 °C and used within the stated shelf life, while unused microplate strips and reference standard aliquots are best stored frozen at -20 °C to preserve activity across repeated freeze-thaw cycles. Researchers generally run duplicate wells per sample, generate a standard curve for interpolation, and express results in ng/mL. Because peripheral BRWD1 abundance is expected to be low, sample concentration or immunoprecipitation enrichment may be required before loading.