Human Bridging Integrator 1 (BIN1) ELISA Kit

BIN1 (Bridging Integrator 1) is a nucleocytoplasmic adaptor protein whose N-BAR membrane-curvature domain and SH3 domain support clathrin-mediated endocytosis, membrane tubulation, and T-tubule organization in myocytes. Tissue expression is highest in skeletal muscle and in the central nervous system, where alternative splicing produces muscle-enriched and brain-enriched isoforms.

The Assay Genie ELISA Kit quantifies human BIN1 in research settings, supporting studies of Alzheimer's disease genetics (BIN1 is the second most strongly associated susceptibility locus for late-onset disease in genome-wide screens), autosomal-dominant centronuclear myopathy (driven by mutations that disrupt the muscle-specific isoform), cardiac remodeling, and BIN1 loss in melanoma or hepatocellular carcinoma models.

Each kit ships with pre-coated microplate wells, biotinylated detection reagent, recombinant calibrators, and substrate solution to run 96 individual BIN1 determinations per plate. Capture antibodies are immobilized on the plate, and bound analyte produces an absorbance change proportional to BIN1 concentration. Reagents should be refrigerated at 4°C upon arrival, protected from light, and the standard curve should be reconstituted immediately before use.

Validated for serum-derived, plasma-derived, and tissue-homogenized preparations from human donors, this ELISA Kit is supplied by Assay Genie for research use only and is not intended for diagnostic procedures.