Human Breast Cancer Anti Estrogen Resistance 1 (BCAR1) ELISA Kit

This quantitative immunoassay from Assay Genie measures Breast Cancer Anti-Estrogen Resistance 1 (BCAR1), also known as p130Cas, in human research samples. BCAR1 is a docking/scaffold signaling protein linked in published breast cancer research to tamoxifen resistance, cell migration, and estrogen-receptor-independent proliferation, making it a relevant biomarker candidate for investigations into anti-estrogen therapy response and downstream signaling pathway analysis.

The kit is supplied as a complete microplate-format package with reagents sufficient for 96 individual determinations, and is validated for use with multiple human specimen types including serum samples, plasma preparations, tissue homogenates, and cell-derived preparations. Detection relies on an enzyme-substrate chromogenic reaction that yields a measurable absorbance signal proportional to BCAR1 concentration in the specimen. Store refrigerated when not in use; standard ELISA wash buffer and substrate working solutions are prepared according to the enclosed protocol insert.

Researchers studying endocrine resistance mechanisms, HER2 and c-Src signaling crosstalk, focal adhesion dynamics, or triple-negative breast cancer pathways will find this BCAR1 ELISA Kit useful for quantifying protein expression in vitro and in cell-based model systems. Standard laboratory ELISA equipment (a plate reader with visible-wavelength absorbance capability, micropipettes, and a wash apparatus) is required but not supplied.