Human BPIFA2/BPI fold-containing family A member 2 ELISA Kit

This sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of human BPIFA2 (BPI fold-containing family A member 2) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Using a double-antibody sandwich format with colorimetric detection, the kit provides a sensitivity of 9.375 pg/mL and a measuring range of 15.625-1000 pg/mL, and components should be stored at 2-8°C for 12 months.

The assay uses a pre-coated microplate, a biotin-labeled detection antibody, and HRP-Streptavidin (SABC) with TMB substrate. Absorbance is read at 450 nm after the reaction is stopped, and signal intensity is proportional to the concentration of the target analyte. Reported linearity at 1:2, 1:4, and 1:8 dilutions ranges from 80-104% across serum, EDTA plasma, and heparin plasma samples, with spike-recovery values averaging 98% across the same sample types.

BPIFA2 is a member of the bactericidal/permeability-increasing protein (BPI) fold-containing family and is involved in immune defense and host-pathogen interactions, with roles in mucosal immunity and inflammatory responses in the respiratory system. The kit is suited to research on respiratory infections, asthma, and chronic obstructive pulmonary disease (COPD), among other conditions.