Human BMSC UbP ELISA Kit (HUFI07984)

This ELISA from Assay Genie is configured for quantitative detection of ubiquitin-like protein markers expressed by human bone marrow stromal cells (BMSC), supporting research into mesenchymal lineage differentiation, hematopoietic niche signaling, and regenerative medicine workflows. The kit is supplied with a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, chromogenic substrate, and stop reagent. Unopened components should be stored refrigerated at 4 degrees C and protected from light until the printed expiration date; reconstituted working solutions are best kept frozen in small aliquots to limit freeze-thaw cycles. Researchers in stem cell biology, orthopedic tissue engineering, and hematology labs use the assay to monitor UbP expression changes during osteogenic, chondrogenic, and adipogenic induction protocols in vitro, with a 100 uL sample volume per well recommended for both standards and unknown specimens. Results are read by absorbance measurement after the enzymatic reaction is stopped, with the standard curve generated from serial dilutions of the supplied calibrator.