Human BMP-5 (Bone Morphogenetic Protein 5) ELISA Kit (HUES02646)

BMP-5 signals through a heteromeric complex of type I (ALK2, ALK3, ALK6) and type II (BMPRII, ActRIIA, ActRIIB) serine/threonine kinase receptors, phosphorylating SMAD1, SMAD5, and SMAD8 transcription factors that translocate to the nucleus and regulate osteoblast and chondrocyte target genes. Researchers studying congenital skeletal malformations, heterotopic ossification, and tendon-bone interface regeneration have used BMP-5 quantification to correlate circulating ligand levels with disease progression. Published work also implicates BMP-5 in neural crest cell migration, dendritic arborization in sympathetic neurons, and pulmonary vascular remodeling. The Assay Genie protocol typically delivers standard curves spanning roughly 31.2 to 2000 pg/ml, with intra-assay precision under 10% CV and inter-assay CV under 15%, and pre-coated monoclonal capture antibodies on the strip-well microplate shorten hands-on time. Each kit contains a 96-well plate, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, TMB substrate, stop solution, and serial-dilution recombinant standards. Reconstituted standards should be aliquoted into single-use vials and stored frozen at -20 degrees C to minimize freeze-thaw degradation, while unopened plates remain stable refrigerated at 2-8 degrees C until the printed expiration date. For research use only.