Human BMP-4 PharmaGenie ELISA Kit (SBRS0312)

Bone Morphogenetic Protein 4 (BMP-4) is a secreted signaling molecule of the transforming growth factor-β superfamily that drives osteoblast differentiation, chondrogenesis, and early mesoderm patterning. This kit from Assay Genie provides quantitative measurement of human BMP-4 across cell culture supernatants, cell lysates, plasma, serum, and tissue lysates using an absorbance-based readout, supporting studies of skeletal biology, stem cell differentiation, and developmental signaling pathways.

Supplied as a 96-assay format kit intended for research use only, each well is coated to capture the mature BMP-4 dimer from prepared samples. Investigators working on bone regeneration, tooth development, neurogenesis, or cancer cell signaling can profile BMP-4 release into conditioned media or compare expression across treated versus untreated cell populations. The protocol accommodates both small-volume samples and standard dilutions, with the absorbance endpoint read on a conventional microplate reader.

Storage of the unopened kit follows the manufacturer's recommendations: sealed components should be held refrigerated and protect from light to preserve antibody binding activity through the stated shelf life. Reconstituted reagents and prepared standard curves should be aliquoted to limit freeze-thaw cycles, which can degrade recombinant BMP-4 and reduce recovery in downstream quantification.