Human BMP-4 PharmaGenie ELISA Kit (SBRS0311)

Bone Morphogenetic Protein 4 (BMP-4) is a secreted signalling molecule in the TGF-β superfamily that drives osteogenic differentiation, chondrogenesis, neural patterning, and pluripotent stem cell fate decisions, and the Assay Genie Human BMP-4 PharmaGenie ELISA Kit delivers quantitative measurement of this cytokine across multiple human-derived specimens. Researchers use the assay to monitor endogenous BMP-4 release in mesenchymal stem cell cultures, embryonic body formation protocols, and bone repair model systems. The kit accepts 100 µL of sample per well and quantifies BMP-4 with sensitivity below 15 pg/mL across a standard curve spanning 31.25 to 2000 pg/mL. Validated matrices include cell culture supernatants, cell lysates, serum specimens, plasma specimens, and tissue lysates. Each microplate produces 96 assays per run, supporting biological and technical replicate panels in regenerative medicine, developmental biology, and skeletal tissue engineering studies. Reagents store refrigerated at 4 °C on arrival, while reconstituted standards and antibody vials are aliquoted and held at -20 °C to preserve activity through repeated freeze-thaw cycles. The complete two-antibody ELISA protocol finishes within 3-4 hours at room temperature and is intended for research use only.