Human BMP-2 PharmaGenie ELISA Kit (SBRS0310)

The BMP-2 (Bone Morphogenetic Protein 2) human immunoassay kit from Assay Genie supports quantitative measurement of this key TGF-beta superfamily cytokine across research samples including cell culture supernatants, plasma, and serum. BMP-2 plays a central role in osteogenic differentiation, cartilage formation, and bone repair pathways, making it a frequent target in stem cell research, fracture healing studies, and skeletal tissue engineering workflows.

Each Assay Genie kit ships as a complete package with reagents for 96 assays in a microplate-based workflow and a colorimetric readout, intended for in vitro research use only. Researchers commonly run standards and unknowns in duplicate to support reliable standard curve interpolation across the dynamic range. The kit is configured for human BMP-2 detection and is used to monitor cytokine secretion in mesenchymal stem cell differentiation models and clinical cohorts investigating skeletal disease.

Standard protocol steps include sample preparation, incubation with biotinylated detection antibody, streptavidin-HRP binding, substrate addition, and absorbance measurement. The microplate layout allows batched processing of experimental replicates and conditioned media samples, supporting efficient workflow integration in laboratories studying bone morphogenetic protein signaling pathways.