Human BIM/BCL2L11 ELISA Kit

Assay Genie provides a sandwich ELISA configured for the quantitative measurement of human BIM/BCL2L11 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The double-antibody sandwich format is read by colorimetric detection, and the assay is referenced to UniProt entry O43521. Synonyms reported for the target include Bcl2L11, BAM, BIM, BOD, BimL, BimEL and BIM Long, and the intended research area is cell death.

Performance characteristics cited by Assay Genie include a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. Spike-and-recovery testing in serum, EDTA plasma and heparin plasma (n=5 each) yielded average recoveries of 96%, 95% and 94% respectively, with individual recovery ranges of 88–103%, 90–102% and 87–105%. Parallelism (linearity) assessed across 1:2, 1:4 and 1:8 dilutions was 93–105%, 86–96% and 84–99% in serum; 89–104%, 82–95% and 82–98% in EDTA plasma; and 85–105%, 82–96% and 83–94% in heparin plasma.

The supplied protocol involves equilibrating reagents, loading standards and samples on the pre-coated plate for a 37°C primary incubation of 90 minutes, followed by sequential incubations with a biotinylated detection antibody (37°C, 60 minutes) and HRP-streptavidin conjugate (37°C, 30 minutes). TMB substrate is then added for 10–20 minutes of colour development in the dark, the reaction is stopped, and absorbance is read at 450 nm. Reagents and the microplate are stored at 2–8°C (with long-term standard storage at –20°C), and the stated shelf life is 12 months.