Human BH3 Interacting Domain Death Agonist (Bid) ELISA Kit

The Bid protein is a BH3-only member of the Bcl-2 family that bridges death-receptor signaling to mitochondrial apoptosis, making this Assay Genie quantification kit valuable for apoptosis-focused research. Researchers in oncology, hepatotoxicity screening, neurodegeneration, and immunology can apply the kit to measure endogenous Bid levels in cell lysates, tissue homogenates, and cell culture supernatants, supporting studies of caspase-8 cleavage, tBid translocation, and mitochondrial outer membrane permeabilization.

The colorimetric readout, measured on a standard microplate reader, delivers quantitative results suitable for time-course experiments, dose-response drug screening, and comparative analyses between treated and control cohorts. Each kit contains pre-coated plates and reagents sufficient for 96 determinations, and components should be refrigerated upon arrival to preserve activity. Compatible with diverse human biofluids and lysate-based matrices, the assay accommodates both clinical workflows and basic cell biology pipelines.

Intended for research use only, the kit integrates easily with orthogonal readouts such as western blot or flow cytometry, allowing researchers to cross-validate Bid expression changes against other apoptotic markers including Bax, Bak, caspase-3, and cytochrome c release.