Human Betatrophin (C19orf80) ELISA Kit

A sandwich ELISA from Assay Genie is designed for the quantitative measurement of human betatrophin (C19orf80) in research samples, with reactivity restricted to human analytes. Validated sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The detection range is 78-5000pg/mL with a reported sensitivity of 32pg/ml.

Precision values supplied by the manufacturer are an intra-assay CV of 6.5% and an inter-assay CV of 9.2%. Recovery is documented for serum (average 101%, range 95-107%) and plasma (average 103%, range 97-109%). Linearity across dilutions from 1:2 to 1:16 is provided for serum (95-107%, 86-99%, 97-107%, 87-96%), EDTA plasma (96-106%, 91-100%, 102-112%, 90-100%) and heparin plasma (92-102%, 103-113%, 86-96%, 83-93%). The assay is specific for natural and recombinant human betatrophin.

Listed synonyms for the target include Angiopoietin-like protein 8, Lipasin, UNQ599/PRO1185 and Angptl8. The protein is described in the manufacturer literature as a hormone that specifically promotes pancreatic beta cell proliferation and beta cell mass expansion, thereby improving glucose tolerance, and also acts as a blood lipid regulator that regulates serum triglyceride levels, possibly by promoting ANGPTL3 cleavage, with which the protein interacts. Subcellular location is given as secreted and the listed research area is immunology. The kit is labelled for research use only.