Human Beta-secretase 2/BACE2 ELISA Kit

A Sandwich ELISA from Assay Genie enables the in vitro quantitative determination of Human Beta-secretase 2 (BACE2) concentrations in serum, plasma, and other biological fluids. The kit has a reported sensitivity of 0.094ng/ml and an assay range of 0.156-10ng/ml. Precision is supported by an intra-assay CV<8% and an inter-assay CV<10%.

Recovery was evaluated by spiking matrices with Human BACE2: serum averaged 95% (range 86-103%), EDTA plasma averaged 96% (range 90-101%), and UFH plasma averaged 96% (range 87-101%). Linearity was tested at 1:2, 1:4, and 1:8 dilutions, with serum showing 90-104% to 88-104%, EDTA plasma 93-101% to 85-98%, and UFH plasma 80-93% to 82-98% of expected concentrations.

Components include an 8×12-strip ELISA microplate, lyophilized standard, sample/standard dilution buffer, biotin-labeled antibody, antibody dilution buffer, HRP-streptavidin conjugate (SABC), SABC dilution buffer, TMB substrate, stop solution, 25X wash buffer, and plate sealer. The kit is stored at 4°C for 6 months, with the lyophilized standard stored at 4°C/-20°C. BACE2 is an aspartic protease responsible for proteolytic processing of amyloid precursor protein (APP), cleaving APP between residues 690 and 691 to release beta-cleaved soluble APP, and can also cleave between residues 671 and 672; it belongs to the peptidase A1 family with 5 isoforms produced by alternative splicing (UniProt Q9Y5Z0). For Research Use Only.