Human Beta-defensin 124/DEFB124 ELISA Kit

Assay Genie offers a Sandwich ELISA Kit designed for the quantitative measurement of human Defensin Beta 124 (DEFb124; synonym DEFB-24; UniProt Q8NES8), a member of the defensin protein family known for antimicrobial activity and roles in innate immunity. The colorimetric assay is read at 450nm and is suitable for serum, plasma, and other biological fluids from human samples.

Performance specifications include a sensitivity of 5.6pg/mL and a working range of 15.625-1000pg/mL. Reported recovery ranges are 83-100% (average 91%) in serum, 90-98% (average 94%) in EDTA plasma, and 90-99% (average 94%) in heparin plasma. Linearity across 1:2 to 1:16 dilutions was demonstrated at 82-96% / 83-98% / 81-99% / 93-101% for serum, 88-101% / 86-95% / 90-102% / 80-93% for EDTA plasma, and 80-91% / 82-90% / 95-104% / 79-95% for heparin plasma. Intra-assay precision is CV <10%, inter-assay precision is CV <12%, and the loss rate of activity is less than 5% within the expiration date under appropriate storage conditions.

The kit has a shelf life of 12 months and is for research use only. The sandwich protocol involves incubation of standards or samples followed by sequential detection reagents, washes, substrate solution, and stop solution, with absorbance measured at 450nm immediately after stopping.