Human Bcl2L11 (Bcl-2 Like Protein 11) CLIA Kit (HUES00391)

Bcl2L11, also known as BIM (Bcl-2 Interacting Mediator of cell death), is a BH3-only pro-apoptotic member of the Bcl-2 protein family that initiates intrinsic mitochondrial apoptosis. This chemiluminescent immunoassay from Assay Genie enables quantitative measurement of human Bcl2L11 in serum, plasma, and other biological fluids with markedly higher sensitivity than colorimetric ELISA, supporting research in oncology, immunology, and programmed cell death.

The kit is supplied as a complete 96-well microplate format with pre-coated capture wells, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, and chemiluminescent substrate. A typical run uses 100 ul of sample per well, with a standard curve spanning pg/mL concentrations and a wider dynamic range than conventional colorimetric methods. Unopened components should be stored at 2-8 °C and protected from light; reconstituted standards are best aliquoted and held at -20 °C, avoiding repeated freeze-thaw cycles to preserve immunoreactivity.

Typical applications include profiling BIM upregulation in tumor lysates and circulating samples after chemotherapeutic challenge, monitoring apoptosis during T-cell activation or cytokine withdrawal studies, screening small-molecule BH3 mimetics and Bcl-2 family inhibitors, and validating transcriptomic findings at the protein level. The chemiluminescent readout reduces background and improves low-end detection, allowing reproducible quantitation across replicate wells for clinical and translational research use only.