Human Bcl-2 (B-cell Leukemia/Lymphoma 2) ELISA Kit (HUES01400)

Assay Genie's sandwich ELISA kit is designed for the precise quantitative measurement of human BCL-2 (B-cell Leukemia/Lymphoma 2) levels in serum, plasma, and cell culture supernatants. BCL-2 is a key regulator of apoptosis, controlling cell survival and resistance to programmed cell death, and its dysregulation is implicated in leukemia, lymphoma, and other cancers, making it a valuable biomarker for research in cancer biology, immunology, and molecular medicine.

The colorimetric assay uses a biotinylated detection antibody and Avidin-Horseradish Peroxidase (HRP) conjugate, with absorbance measured at 450 nm ± 2 nm. The kit has a sensitivity of 0.1 ng/mL and a detection range of 0.16-10 ng/mL. Recovery is reported at 80%-120%. The recommended sample volume is 100 μL, and total assay time is 3 h 30 min. The kit specifically recognizes human Bcl-2 with no significant cross-reactivity or interference from analogues observed (UniProt ID: P10415). Provided components include a dismountable microplate pre-coated with capture antibody, reference standard, concentrated biotinylated detection antibody (100×), concentrated HRP conjugate (100×), diluents, concentrated wash buffer (25×), substrate reagent, stop solution, plate sealer, technical manual, and certificate of analysis.

Validated performance includes linearity in serum, EDTA plasma, and cell culture media at dilutions from 1:2 to 1:16, with average percentage recoveries ranging from 89% to 105% depending on matrix and dilution. Spike-and-recovery testing showed EDTA plasma recovery of 86-96% (average 91%) and cell culture media recovery of 92-105% (average 100%). Intra-assay precision CV ranged from 4.57% to 6.8% across three sample concentrations, while inter-assay precision CV ranged from 6.78% to 8.74%. The kit is intended for research applications in cancer, cell biology, metabolism, and signal transduction, with storage at 2–8°C.