Human BCDIN3D (Pre-miRNA 5?-monophosphate methyltransferase) ELISA KIT (HUFI05425)

BCDIN3D (Bicoid-Interacting Protein 3 Domain-Containing, also annotated as BCDIN3) is a 2'-O-methyltransferase that caps the 5' monophosphate of precursor microRNAs, protecting pre-miRNA intermediates from exonucleolytic trimming and modulating miRNA maturation. The Assay Genie kit targets the human protein and is supplied in a microplate configured for 96 Assays, validated specifically for human specimens with no significant cross-reactivity against rodent Bcdin3d orthologs.

Typical applications include quantification of BCDIN3D abundance in translational research on miRNA biogenesis, post-transcriptional gene regulation, and cancer biomarker discovery, particularly in hepatocellular carcinoma, breast cancer, and acute myeloid leukemia models where altered BCDIN3D expression has been documented. Reconstituted standards and detection antibody should be aliquoted and stored frozen at -20 degrees C, while the sealed kit is shipped refrigerated. For research use only, the assay supports in vitro studies correlating BCDIN3D protein levels with downstream miRNA processing capacity in liquid biopsy and other clinical specimens.