Human Bag3/Bcl2 Associated Athanogene 3 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the precise quantitative measurement of human BAG3 (Bcl-2-associated athanogene 3) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a double-antibody sandwich format with colorimetric detection and absorbance read at 450 nm.

BAG3 is a protein that interacts with the anti-apoptotic protein Bcl-2 and plays a key role in regulating cell survival and apoptosis. Its dysregulation has been implicated in cancer, neurodegenerative disorders, and cardiovascular conditions, making it a biomarker of interest for disease progression and a potential therapeutic target. Reagents are stored at 2-8°C for 12 months.

Reported linearity in serum (n=5) is 85-103% (1:2), 96-101% (1:4), and 83-99% (1:8); in EDTA plasma (n=5) 89-101% (1:2), 92-101% (1:4), and 88-98% (1:8); and in heparin plasma (n=5) 87-104% (1:2), 89-101% (1:4), and 81-97% (1:8). Recovery averages 97% in serum (range 91-104%), 95% in EDTA plasma (range 90-102%), and 95% in heparin plasma (range 85-104%). The associated Uniprot ID is O95817, and listed synonyms include BAG3, BAG-3, BIS, CAIR-1, and BAG family molecular chaperone regulator 3.