Human Baculoviral IAP repeat-containing protein 5 (BIRC5) ELISA Kit

A high quality Sandwich ELISA Kit from Assay Genie designed to quantify human Baculoviral IAP repeat-containing protein 5 (BIRC5), also known as survivin, Apoptosis inhibitor 4, API4 and IAP4. The assay targets BIRC5, a multitasking protein with dual roles in promoting cell proliferation and preventing apoptosis, and a member of the inhibitor of apoptosis (IAP) gene family whose overexpression has been linked to poor prognosis in various cancers, making it a valuable biomarker for cancer research. The kit is validated for use with human samples including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and shows specificity for natural and recombinant human BIRC5.

Performance characteristics reported by the manufacturer include a detection range of 0.156–10 ng/mL and a sensitivity of 0.078 ng/mL. Intra-assay CV is 6.4% and inter-assay CV is 10.8%. Linearity in serum (n=5) was 101–113% at 1:2, 105–115% at 1:4, 102–112% at 1:8 and 103–114% at 1:16; in EDTA plasma (n=5) 110–120%, 81–91%, 92–102% and 87–99% across the same dilutions; and in heparin plasma (n=5) 108–119%, 109–117%, 106–118% and 101–111%. Spike-and-recovery averaged 108% (range 102–114) for serum and 110% (range 104–116) for plasma.

The kit is intended for research use only, supporting investigations into the role of BIRC5 in cancer biology and other areas of human health and disease.