Human Bactericidal/Permeability Increasing Protein (BPI) ELISA Kit

Bactericidal/Permeability-Increasing Protein (BPI) is a ~55 kDa cationic antimicrobial polypeptide stored in the azurophilic granules of human polymorphonuclear leukocytes, where it contributes to innate host defense by disrupting the outer membranes of gram-negative bacteria and neutralizing lipopolysaccharide (LPS) endotoxin.

This Assay Genie ELISA kit provides a quantitative immunoassay for measuring human BPI in research samples, supporting investigations into neutrophil biology, sepsis, endotoxin-driven inflammation, and antimicrobial peptide activity. The kit delivers a colorimetric readout and is supplied as a complete 96-assay format with pre-coated microplate wells, biotinylated detection antibody, streptavidin-HRP conjugate, substrate solution, wash buffer, stop reagent, and a recombinant human BPI standard for curve generation.

Room-temperature incubation steps and a standard wash protocol keep the workflow practical for routine bench use, and the kit is intended for research use only, not for clinical diagnostic applications. Researchers studying gram-negative bacteremia, cystic fibrosis airway inflammation, meningococcal disease, or modulation of the LPS response will find this assay a focused addition to their experimental toolkit.