Human B7-H3/CD276 PharmaGenie ELISA Kit (SBRS0293)

B7-H3 (CD276) is a type I transmembrane glycoprotein in the B7 family of immunomodulatory molecules, and the Assay Genie Human B7-H3/CD276 PharmaGenie ELISA Kit provides a quantitative immunoassay for measuring this checkpoint molecule in human research samples. Because B7-H3 is frequently overexpressed in solid tumors such as prostate, ovarian, lung, and pancreatic cancers, this assay supports investigations in tumor immunology, immune checkpoint biology, and cancer immunotherapy research.

This microplate-based immunoassay accepts cell culture supernatants, plasma, and serum, allowing workflows that bridge in vitro cell-based experiments with translational clinical sample analysis. The colorimetric method enables quantification using standard laboratory plate readers, and the kit is supplied as a 96-assay microplate format suitable for moderate-throughput screening panels. Reagents should be kept refrigerated between uses to preserve calibration curve integrity and antibody binding performance.

Researchers studying tumor microenvironment profiling, biomarker discovery, and treatment response correlations will find the kit useful for characterizing B7-H3 expression levels across experimental conditions. Each kit provides the pre-coated microplate, detection antibodies, and standard reference reagents needed to run the standard curve and unknown samples, with results interpolated in concentration units against the recombinant human B7-H3 reference standard.