Human B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) ELISA Kit

The Human B-Raf Proto Oncogene Serine/Threonine Protein Kinase ELISA Kit from Assay Genie enables quantitative measurement of BRAF, a serine/threonine kinase that operates within the MAPK/ERK signaling cascade. BRAF acts upstream of MEK and ERK, and activating mutations such as V600E produce constitutive pathway signaling implicated in melanoma, colorectal, and thyroid malignancies. Investigators apply this ELISA to quantify BRAF protein levels in cell lysates and tissue homogenates, supporting studies of oncogenic pathway activity, small-molecule inhibitor response, and downstream effects of genetic manipulation. The assay is validated for human BRAF and uses 100 µL of sample per well in a microplate-based format that works with standard absorbance plate readers and is amenable to medium-throughput screening campaigns. Reagents should be stored at 4°C; protect from light and limit freeze-thaw cycles to maintain capture antibody performance through the assay window.