Human B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

Mitochondrial Bcl-2 quantification in human research samples supports studies of programmed cell death, lymphoma biology, and chemoresistance mechanisms. Bcl-2 is a 26 kDa anti-apoptotic protein anchored in the outer mitochondrial membrane, where it restrains the intrinsic apoptosis pathway by sequestering pro-apoptotic factors such as Bax and Bak. Loss of Bcl-2 restraint is central to follicular lymphoma, chronic lymphocytic leukemia, and several solid tumor types where malignant cells evade programmed cell death.

This 96 assays ELISA kit from Assay Genie accommodates serum samples, plasma, tissue homogenates, cell lysates, and cell culture supernatants from human donors. Workflows supported include BH3 mimetic screening with agents such as venetoclax, characterization of mitochondrial outer membrane permeabilization, evaluation of chemoresistance in hematological malignancies, and investigation of Bcl-2-dependent cardioprotective or neuroprotective signaling. Researchers working on diffuse large B-cell lymphoma, multiple myeloma, or autoimmune disease models can integrate Bcl-2 measurements alongside western blot and flow cytometry endpoints.

Performed as an in vitro research use only assay, the kit provides core reagents to establish a standard curve and process samples on a microplate. Colorimetric readout enables quantification on standard laboratory plate readers, and results can be cross-validated by immunohistochemistry, PCR-based expression profiling, or functional apoptosis assays downstream.