Human AVPR1b/Vasopressin V1b receptor ELISA Kit

Designed for the accurate detection of AVPR1B (Vasopressin V1B Receptor) levels in human biological samples, this Sandwich ELISA Kit from Assay Genie uses a double-antibody format with colorimetric readout at 450 nm. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, supporting research into AVPR1B signaling pathways relevant to stress response, social behavior, anxiety, depression, and autism spectrum disorders.

Performance specifications include a sensitivity of 0.188 ng/mL and a detection range of 0.313-20 ng/mL. Reagents are stable at 2-8°C for 12 months. Linearity across 1:2, 1:4, and 1:8 dilutions for serum, EDTA plasma, and heparin plasma (n=5 each) falls within 81-105%, while recovery ranges from 88-104% (averages of 96% for serum, 97% for EDTA plasma, and 92% for heparin plasma).

The protocol covers reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding (60 minutes), HRP-Streptavidin incubation (30 minutes), TMB color development in the dark for 10-20 minutes, and a stop reaction measured at 450 nm. Sample preparation notes are provided for serum, plasma, tissue homogenate, cell culture supernatant, and cell lysate.