Human AVPR V1a/Vasopressin V1a receptor ELISA Kit

From Assay Genie, this Sandwich ELISA, Double Antibody assay is designed for human samples and quantitatively measures the AVPR V1a/Vasopressin V1a receptor. The receptor has been described as a key player in the vasopressin signaling pathway influencing vasoconstriction, water reabsorption, and social behavior, with dysregulation implicated in hypertension, heart failure, and mood disorders. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is categorized for Cell Biology research and is associated with UniProt entry P37288.

The kit reports a sensitivity of 37.5 pg/mL and a detection range of 62.5-4000 pg/mL, with absorbance read at 450 nm. Storage is 2-8°C for 12 months.

The assay procedure follows a six-step workflow: reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding at 37°C for 60 minutes, HRP-Streptavidin (SABC) binding at 37°C for 30 minutes, TMB color development in the dark for 10–20 minutes, and stop reaction with absorbance measurement. Reported linearity at 1:2, 1:4, and 1:8 dilutions for serum (n = 5) spans 80–105%, EDTA plasma (n = 5) spans 83–101%, and heparin plasma (n = 5) spans 83–104%. Recovery ranges are 85–96% (serum, average 91%), 88–96% (EDTA plasma, average 91%), and 85–99% (heparin plasma, average 93%).