Human AVP/Vasopressin-neurophysin 2-copeptin ELISA Kit

This Competitive ELISA kit from Assay Genie is designed for the quantitative measurement of human AVP/vasopressin-neurophysin 2-copeptin using a sandwich double-antibody detection format with colorimetric readout. The kit is reactive for human samples and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Performance characteristics include a detection range of 15.625–1000 pg/mL and a sensitivity of 9.375 pg/mL. Linearity for serum, EDTA plasma, and heparin plasma (n=5) at 1:2, 1:4, and 1:8 dilutions is reported as 87–105%, 85–99%, and 85–104% respectively across the dilution series. Recovery in serum averages 96%, EDTA plasma 94%, and heparin plasma 97%, with corresponding ranges of 89–100%, 86–100%, and 86–103%. The kit should be stored at 2–8°C for up to 12 months. Synonyms listed include AVP, Vasopressin-neurophysin 2-copeptin, ARVP, VP, and AVP-NPII, with Uniprot accession P01185.

The research area is neuroscience, and the assay targets a hormone involved in regulating water retention, blood pressure, and social behaviors that plays a role in conditions such as diabetes insipidus, septic shock, and heart failure. The procedure involves reagent equilibration, a primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB substrate development in the dark for 10–20 minutes, and absorbance measurement at 450 nm after stop solution addition.