Human Autophagy Related Protein 3 (ATG3) ELISA Kit

Quantify human autophagy-related protein 3 (ATG3) using this immunoassay from Assay Genie. ATG3 acts as an E2-like conjugating enzyme essential for autophagy, transferring activated LC3 onto phosphatidylethanolamine during autophagosome biogenesis, so measuring its abundance is a useful readout for pathway activity in cell biology and disease-model research.

The kit is configured for use with tissue homogenates, cell lysates, and similar human-derived research samples. Each package includes a pre-coated microplate, biotinylated detection reagent, streptavidin-enzyme conjugate, a set of calibrators, wash buffer, and assay diluent sufficient for the stated assay count. A compatible plate reader is required for signal capture and data reduction.

Reconstituted components should be stored according to the insert and handled with standard laboratory precautions to protect from contamination; the kit is intended for research use only and has not been validated for diagnostic procedures. Curve fitting is performed against the supplied calibrators, and unknown sample concentrations are interpolated from the resulting standard curve.