Human Autophagy Related Protein 12 (ATG12) ELISA Kit

Quantify endogenous ATG12 with this enzyme-linked immunosorbent assay from Assay Genie, supplied as a kit configured for 96 assays. ATG12 operates through ubiquitin-like covalent conjugation to ATG5, and the resulting ATG12–ATG5 conjugate associates with ATG16L1 to promote autophagosome membrane elongation during macroautophagy. Measuring ATG12 abundance supports research on nutrient deprivation responses, ER stress, mitophagy in neurodegeneration models, tumor cell survival under hypoxia, and host–pathogen interactions involving intracellular bacteria or viruses. The plate-based format is applied to cell lysates and tissue homogenates prepared from cultured human lines or biopsied material, enabling both baseline expression studies and perturbation experiments where autophagy flux is chemically blocked or induced. Researchers commonly pair ATG12 readouts with LC3-II turnover and p62/SQSTM1 accumulation data to characterize autophagic activity in a given model. Reagents are supplied in vials and a well plate with standard buffers; kit components should be stored refrigerated for short-term use and frozen at -20 °C for longer-term storage to preserve antibody and standard stability. Assay Genie provides this kit for research use only, intended for laboratory investigation of human autophagy pathways.