Human Atrial natriuretic peptide receptor 2/NPR2 ELISA Kit

A sandwich ELISA, double-antibody kit from Assay Genie for the quantitative measurement of Human Atrial natriuretic peptide receptor 2 (NPR2) in human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a colorimetric detection method and is intended for cardiovascular research, where NPR2 is implicated in atrial natriuretic peptide (ANP) signalling and conditions such as hypertension and heart failure.

The kit reports a sensitivity of 1.5 ng/mL and a detection range of 2.5–160 ng/mL, with reactivity confirmed for human samples. Linearity for serum, EDTA plasma and heparin plasma falls within 80–103% across 1:2, 1:4 and 1:8 dilutions. Recovery averages 96% for serum, 95% for EDTA plasma and 99% for heparin plasma. Components include a pre-coated dismountable microplate, lyophilized standard, biotin-labelled detection antibody, HRP-Streptavidin conjugate (SABC), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, 25× wash buffer and plate sealers. The standard assay procedure involves a 90-minute primary incubation at 37 °C, a 60-minute detection antibody step, a 30-minute HRP-Streptavidin incubation, 10–20 minutes of TMB colour development in the dark, and absorbance reading at 450 nm after stop solution is added. Storage is at 2–8 °C for 12 months, with lyophilized standard and microplate strips also storable at –20 °C for 12 months.