Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) ELISA Kit

Encoded by the ATP1A1 gene, the alpha-1 catalytic subunit of the Na+/K+-ATPase is a ubiquitous P-type ATPase that maintains the electrochemical sodium and potassium gradients driving secondary active transport, resting membrane potential, and cell volume regulation in virtually every mammalian tissue. This quantitative immunoassay from Assay Genie is calibrated for human antigen and is supplied as a 96-assay research kit for in vitro measurement in tissue homogenates.

Investigators studying cardiac, renal, and neurological function rely on accurate quantitation of alpha-1 expression to explore the cardiotonic steroid hypothesis, ischemia-reperfusion injury, and salt-sensitive hypertension models. The kit supports research into how endogenous ouabain-like compounds and other cardiotonic steroids modulate pump activity, as well as downstream effects on intracellular calcium handling through the Na+/Ca2+ exchanger. Applications extend to studies of nephron function in kidney cortex, neuronal excitability in brain lysates, and contractile remodeling in cardiac tissue preparations.

Each Assay Genie kit is configured for laboratory research use only and supplies the reagents and consumables required to perform the full 96-assay run, with absorbance read on a standard plate reader. The procedure tolerates the detergent and salt conditions typical of membrane-fraction preparations, supporting work on alpha-1 subunit abundance in subcellular fractions where the sodium pump is known to partition.