Human ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) ELISA Kit

The H+/K+ ATPase beta subunit functions as the glycoprotein partner of the gastric proton pump, anchoring the catalytic alpha subunit and supporting its trafficking to the apical membrane of parietal cells in the stomach lining, where it powers hydrochloric acid secretion. This quantitative immunoassay from Assay Genie enables researchers to measure ATP4B protein levels in human samples for studies of peptic ulcer disease, gastroesophageal reflux pathology, Helicobacter pylori-driven gastric inflammation, and the pharmacodynamics of proton pump inhibitor drugs such as omeprazole.

The kit includes pre-coated plate strips and all reagents required to produce a standard curve from prepared tissue lysates, with absorbance read on a standard microplate reader. Researchers can apply the protocol to lysed tissue preparations and cell extracts generated through standard homogenization and centrifugation steps. Each kit provides materials for 96 individual determinations, supporting both full-plate runs and smaller batch experiments across multiple sample sets.

Reagents should be kept refrigerated on arrival and equilibrated to working temperature before use. The complete protocol, diluent buffers, and consumables needed to perform the assay are included in the box, with no specialized equipment required beyond a plate reader capable of measuring absorbance in the visible range.