Human ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit

ATP2B2 encodes PMCA2, a P-type plasma membrane calcium-transporting ATPase responsible for extruding cytosolic Ca²⁺ into the extracellular space. This 96-assay kit from Assay Genie provides a quantitative immunoassay for measuring human ATP2B2 protein in tissue homogenates and cell lysates, supporting research into calcium-signaling pathways, cochlear hair-cell function, and neuronal calcium homeostasis where PMCA2 isoform activity is known to play a critical role.

The configuration uses paired capture and detection antibodies immobilized on a microplate, with readout generated by an enzyme-substrate reaction. Reconstituted standard is serially diluted to generate a calibration curve, allowing interpolation of unknown samples run in parallel. Reagents are supplied as a complete pack sized for 96 determinations per kit.

Validated for human reactivity, the kit accepts lysate inputs prepared from cultured cells or freshly harvested tissue using standard detergent-based lysis buffers. Researchers studying PMCA2 isoform expression in auditory, cerebellar, or mammary tissue can compare lysates against the supplied standard curve to obtain relative target concentrations. Unopened kits should be kept refrigerated until first use; reconstituted reagents should be aliquoted to limit freeze-thaw cycles and stored at the temperature indicated on the certificate of analysis.