Human ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ELISA Kit

The ATP2A2/SERCA2 quantitative immunoassay from Assay Genie targets the human cardiac slow-twitch calcium ATPase encoded by the ATP2A2 gene. SERCA2 is the sarcoplasmic reticulum Ca²⁺-ATPase responsible for re-sequestering calcium into the SR during diastole, a process central to cardiac muscle relaxation and to the contraction-relaxation cycle of slow-twitch skeletal fibers. The kit uses an absorbance-based readout on a microplate and is validated for human tissue homogenates and cell lysates, supporting studies of calcium-handling biology in cardiomyocytes and other ATP2A2-expressing cell models. Reagents are supplied in a 96-assay kit format intended for research use only and should be refrigerated upon arrival; allow sealed components to equilibrate to room temperature before running the assay. Investigators studying SERCA2 dysregulation in heart failure, Darier disease (a skin disorder caused by ATP2A2 loss-of-function mutations), or slow-twitch skeletal muscle physiology can apply the kit to compare SERCA2 protein abundance across experimental conditions.