Human ATP synthase subunit beta, mitochondrial (ATP5B) ELISA Kit

Assay Genie offers a Sandwich ELISA Kit for the quantitative measurement of human ATP synthase subunit beta, mitochondrial (ATP5B), a critical subunit of mitochondrial ATP synthase (also referred to as F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane. The kit uses a colorimetric detection method, is reactive against human analyte, and is intended for research use only. Dysregulation of ATP5B has been implicated in metabolic disorders, neurodegenerative diseases and cancer, supporting its relevance as a biomarker in studies of mitochondrial function and ATP metabolism.

Performance characteristics include a detection range of 0.156-10ng/mL and a sensitivity of 0.084ng/mL. Intra-assay CV is 5.2% and inter-assay CV is 8.3%.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Specificity covers natural and recombinant human ATP synthase subunit beta, mitochondrial (UniProt P06576). The kit is classified within the research area of Neurosciences, with the target localised to the mitochondrion inner membrane as a peripheral membrane protein on the matrix side.