Human ATP-dependent RNA helicase DDX42 (DDX42) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit enables quantitative detection of human ATP-dependent RNA helicase DDX42 in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The assay uses a colorimetric detection method and is intended for research use only, with specificity for both natural and recombinant forms of the human protein.

Reported performance characteristics include a detection range of 78-5000pg/mL and a sensitivity of 34pg/ml. Intra-assay precision is 4.8% and inter-assay precision is 7.4%. Linearity has been demonstrated in serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16. Mean recovery is 98% in serum (range 92-104%) and 100% in plasma (range 94-106%).

DDX42 functions as an ATP-dependent RNA helicase that binds partially double-stranded RNAs to unwind secondary structures, a process promoted by single-strand binding proteins. The protein also mediates RNA duplex formation, displacing single-strand RNA binding proteins, and its activity is modulated by ATP and ADP: ATP binding and hydrolysis drive RNA strand separation, while the ADP-bound form promotes annealing of complementary strands. DDX42 is a component of the SF3B splicing complex and contributes to RNA splicing, translation, and broader RNA metabolism, making it a relevant target for research into RNA biology and associated disease mechanisms.