Human ATP-citrate synthase (ACLY) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative detection of ATP-citrate synthase (ACLY) in human samples, including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. ACLY, also known as ATP-citrate (pro-S-)-lyase, citrate cleavage enzyme, or ACL, is a key enzyme involved in the citrate cycle and fatty acid synthesis, with a central role in de novo lipid synthesis and, in nervous tissue, the biosynthesis of acetylcholine. The kit is intended for research use only and supports studies of metabolic disorders, cancer, and related disease mechanisms, with cardiovascular listed as a relevant research area.

The colorimetric assay provides a detection range of 1.56-100U/mL with a sensitivity of 0.35U/ml. Reported precision values are an intra-assay CV of 3.9% and an inter-assay CV of 8.0%. Specificity covers natural and recombinant human ATP-citrate synthase (UniProt P53396), a homotetramer located in the cytoplasm. The sandwich format uses a microplate-based workflow with a 450 nm read-out following TMB substrate development.