Human ATF2/Activating Transcription Factor 2 ELISA Kit

A high-quality Sandwich ELISA Kit from Assay Genie, designed for the quantitative measurement of Activating Transcription Factor 2 (ATF2) in human samples. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a double-antibody sandwich format with colorimetric detection, and absorbance is measured at 450 nm. Sensitivity is 9.375 pg/mL and the assay range is 15.625-1000 pg/mL.

ATF2 is a transcription factor that regulates gene expression in response to cellular stress signals and contributes to cell growth, differentiation, and survival, making it a relevant biomarker for research into cancer, inflammation, and neurodegenerative disease (Synonym: ATF-2; UniProt: P15336; Research Area: Epigenetics and Nuclear Signaling). The kit is supplied with components for the standard sandwich ELISA workflow, including a pre-coated dismountable microplate, lyophilized standard, biotin-labeled detection antibody (100X), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, 25X wash buffer, plate sealers, and a technical manual.

Performance is documented across serum, EDTA plasma, and heparin plasma. Linearity ranges span 80-99% to 86-102% across the three matrices at n=5 per matrix, and recovery averages are 96% for serum, 99% for EDTA plasma, and 95% for heparin plasma. The kit should be stored at 2-8°C and is stable for 12 months under recommended conditions.