Human ATF2 (Activating Transcription Factor 2) ELISA Kit (HUES01718)

ATF2 (Activating Transcription Factor 2) is a member of the ATF/CREB family of basic leucine zipper transcription factors that regulates gene expression in response to stress signals, DNA damage, and cytokine stimulation. Phosphorylation by JNK and p38 MAPK pathways drives its nuclear accumulation and transcriptional activity, making it a focal point in studies of inflammation, oncogenesis, and cellular stress responses.

This Sandwich ELISA from Assay Genie is built on a 96-well microplate pre-coated with a capture antibody specific for human ATF2. Standards and samples are incubated in the wells, followed by a biotinylated detection antibody and streptavidin-HRP conjugate. A colorimetric TMB substrate is added, producing a measurable absorbance that is proportional to ATF2 concentration. The standard curve supports quantitative determination across the analytical range in serum, plasma, and other biological fluids.

Key features:

• Detection method: Colorimetric, read at 450 nm after stop solution
• Format: 96-well microplate with single-use break-apart strips
• Sample volume: 100 µL per well
• Storage: 2-8 °C short-term; freeze at -20 °C for long-term; protect from light
• Applications: quantitative measurement of ATF2 in cell lysates, tissue extracts, serum, and plasma for research use only