Human Ataxin 1 ELISA Kit

From Assay Genie, this is a sandwich ELISA, double-antibody assay designed for the quantitative measurement of human Ataxin-1 in research samples. The kit falls within the Epigenetics and Nuclear Signaling research area and is associated with Uniprot entry P54253 (synonym ATXN1), supporting investigations of neurological disorders including spinocerebellar ataxia type 1 (SCA1).

Manufacturer-stated specifications include a sensitivity of 0.188 ng/mL and an assay range of 0.313–20 ng/mL. The assay is reactive with human samples and validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reagents should be stored at 2–8°C for 12 months, and absorbance is read at 450 nm after TMB color development.

Reported linearity at 1:2, 1:4 and 1:8 dilutions (n = 5) is 84–99% / 81–91% / 81–90% for serum, 82–97% / 86–98% / 84–101% for EDTA plasma, and 83–99% / 84–99% / 86–90% for heparin plasma. Average spiked recovery is 96% in serum (range 86–104%), 99% in EDTA plasma (range 92–105%) and 98% in heparin plasma (range 91–105%). The procedure includes a 90-minute primary incubation at 37°C, a 60-minute biotinylated detection antibody step, a 30-minute HRP-streptavidin binding step, and TMB color development in the dark for 10–20 minutes.