Human ASTL (Astacin-like metalloendopeptidase) ELISA Kit

Designed for the quantitative measurement of ASTL (Astacin-like metalloendopeptidase) in human samples, this Sandwich ELISA kit from Assay Genie uses a double-antibody configuration and colorimetric detection, with absorbance read at 450 nm. The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and the components are stored at 2-8°C for 12 months. ASTL is associated with extracellular matrix remodeling and cell migration, making it a relevant biomarker for research in oncology, inflammatory disorders, and tissue regeneration.

The protocol includes primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB color development in the dark for 10–20 minutes, and a stop reaction before absorbance is measured at 450 nm. Sample preparation guidance covers serum, plasma, tissue homogenates, cell culture supernatants, and cell lysates, with additional technical support available from Assay Genie for other sample types.