Human Aspartate Aminotransferase (AST) ELISA Kit

Aspartate aminotransferase (AST) is a pyridoxal-phosphate-dependent transaminase widely measured in research settings as a circulating marker of hepatocellular injury and myocardial cell stress. The Human AST ELISA Kit from Assay Genie provides a quantitative immunoassay workflow for assessing AST concentrations in human specimens, supporting investigative work in hepatology, toxicology, and cardiovascular biomarker studies.

The kit ships with reagents in individually sealed vials, a precoated microplate, and printed protocol documentation, sized to run a full plate of determinations in a single batch. The assay follows standard ELISA principles, with results read against a calibrated standard curve generated from serial dilutions of the supplied reference material. Researchers commonly apply this approach when correlating AST levels with alanine aminotransferase (ALT) ratios, evaluating drug-induced liver injury models, or profiling enzyme release following ischaemic challenge.

Reagent components should be refrigerated upon receipt and protected from light until first use to preserve activity. Reconstituted standards and working solutions remain stable through multiple freeze-thaw cycles when aliquoted and stored in line with the manual. The kit is intended for research use only and is not validated for standalone clinical diagnostic reporting.